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BrightLine® single-band filter set, optimized for CFP and other like fluorophores, illuminated with LED-based light sources

Semrock Part Number: LED-CFP-A-ZHE-ZERO

IDEX Part Number: S-000318  /

Semrock

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring CFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,463.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
BD Horizon V500 410 500
BOBO™-1 461 484
Cerulean 434 473
CFP (ECFP) 433 475
DY-415 418 470
ECFP 435 475
evoglow-Bs1 449 496
LysoSensor Green 448 502
mBBr+GSH 394 490
mTFP1 462 492
POPO-1 433 457
PO-PRO-1 433 456
SpectrumAqua 434 481
SYTO 45 451 485
SYTOX Blue 444 470
TagCFP 458 480

Related Products

FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.