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BrightLine® Pinkel filter set, optimized for Green Fluorescent Protein & DsRed and other like fluorophores

Semrock Part Number: GFP/DsRed-2X-A-000

IDEX Part Number: S-001054  /

Semrock

This 4-filter "Pinkel" set is perfectly optimized for imaging GFP and DsRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among other fluorophore combinations. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,675.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-470/22-25

FL-004595

Each
  • Tavg > 93% 459 – 481 nm
  • Center Wavelength of 470 nm and GMBW of 22 nm
  • FWHM of 27 nm

FL-004595

FF01-512/630-25

FL-004381

Each
  • Tavg > 90% 500.5 – 523.5 nm & Tavg > 90% 584.5 – 675.5 nm
  • Center Wavelengths of 512 nm & 630 nm and GMBWs of 23 nm & 91 nm

FL-004381

FF01-556/20-25

FL-004552

Each
  • Tavg > 93% 546 – 566 nm
  • Center Wavelength of 556 nm and GMBW of 20 nm
  • FWHM of 25 nm

FL-004552

FF493/574-Di01-25x36

FL-006972

Each
  • Dichroic Beamsplitter with edges at 493 nm & 574 nm
  • Ravg > 95% 456 – 480 nm & Ravg > 95% 541 – 565 nm
  • Tavg > 90% 500 – 529 nm & Tavg > 90% 584 – 679 nm

FL-006972

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

GFP/DsRed-2X-A-NQF

S-001557 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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GFP/DsRed-2X-A-NTE

S-000254 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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GFP/DsRed-2X-A-OMF

S-001559 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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GFP/DsRed-2X-A-ZHE

S-000255 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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GFP/DsRed-2X-A-OFF

S-001558 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO™ option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 546 556 572
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BCECF (pH 5.5) 481 518
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Orange 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
CFP (ECFP) 433 475
CFP2 490 510
ChlorophyllA 480 650
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
ECFP 435 475
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LOLO-1 568 580
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
PerCP 490 677
pHrodo™, succinimidyl ester