This 5-filter "Sedat" set is ideal for high-speed sequential imaging GFP and DsRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among o
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FF01-514/30-25
FL-003986 / 0
- Tavg > 93% 499 – 529 nm
- Center Wavelength of 514 nm and GMBW of 30 nm
- FWHM of 36 nm
FF02-617/73-25
FL-004462 / 0
- Tavg > 90% 580.5 – 653.5 nm
- Center Wavelength of 617 nm and GMBW of 73 nm
- FWHM of 79 nm
FF01-556/20-25
FL-004552 / 0
- Tavg > 93% 546 – 566 nm
- Center Wavelength of 556 nm and GMBW of 20 nm
- FWHM of 25 nm
FF01-470/22-25
FL-004595 / 0
- Tavg > 93% 459 – 481 nm
- Center Wavelength of 470 nm and GMBW of 22 nm
- FWHM of 27 nm
FF493/574-Di01-25x36
FL-006972 / 0
- Dichroic Beamsplitter with edges at 493 nm & 574 nm
- Ravg > 95% 456 – 480 nm & Ravg > 95% 541 – 565 nm
- Tavg > 90% 500 – 529 nm & Tavg > 90% 584 – 679 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
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| DDAO | 648 | 657 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiI | 551 | 569 |
|
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| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
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| dTomato | 556 | 582 |
|
|
| DY-590 | 582 | 599 |
|
|
| DyLight 594 | 593 | 618 |
|
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| ECFP | 435 | 475 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| mCherry | 587 | 610 |
|
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| Merocyanine 540 | 559 | 579 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mKate2 | 588 | 633 |
|
|
| mOrange | 546 | 562 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Orange 552 | 552 | 574 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| POPO-3 | 533 | 574 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine Green | 497 | 523 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| rsGFP (red shifted GFP, S65T) | 498 | 516 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|