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BrightLine® Sedat filter set, optimized for Green Fluorescent Protein & DsRed and other like fluorophores

Semrock Part Number: GFP/DsRed-2X2M-C-NQF

IDEX Part Number: S-001563  /

Semrock

This 5-filter "Sedat" set is ideal for high-speed sequential imaging GFP and DsRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among o

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,344.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBO™-1 461 484
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Orange 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
CFP (ECFP) 433 475
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
Cy3™ 554 566
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
DDAO 648 657
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiI 551 569
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
ECFP 435 475
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LOLO-1 568 580
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mOrange 546 562
mOrange2 551 565
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
Oregon Green™ 488 498 526
pHrodo™, succinimidyl ester 560 587
PicoGreen® 502 522
PKH67 488 500
POPO-3 533 574
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 525 Nanocrystals 300 525
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine 110 497 519
Rhodamine Green 497 523
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
Rhodol Green 497 524
R-phycoerythrin (PE) 565 576
rsGFP (red shifted GFP, S65T) 498 516
sgGFP™ (super glow GFP) 472 506
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYBR Green I nucleic acid gel stain-DNA 498 522
SYTO 13 448 506
SYTO 16 489 520
SYTO 45 451 485
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
SYTOX Orange 547 570
TagCFP 458 480
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red-X® 595 613
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboGFP 482 503
TurboRFP 553 573
wtGFP (wild type GFP, non-UV excitation) 474 509
X-Rhod-1 Indicator 580 601
YO-PRO-1 491 506
YOYO-1 491 508

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)

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