This filter set is optimized to be the brightest filter set available for measuring GFP and its variants.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,478.64 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-466/40-25
FL-004477 / 0
- Tavg > 93% 446 – 486 nm
- Center Wavelength of 446 nm and GMBW of 40 nm
- FWHM of 45.3 nm
FF03-525/50-25
FL-004656 / 0
- Tavg > 93% 500 – 550 nm
- Center Wavelength of 525 nm and GMBW of 50 nm
- FWHM of 55.9 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| AmCyan1 | 458 | 489 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
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| BOBO™-1 | 461 | 484 |
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| Calcein | 494 | 514 |
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| Cerulean | 434 | 473 |
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| CFP (ECFP) | 433 | 475 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| CyQuant Cell Proliferation Assay | 502 | 523 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
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| DiA | 457 | 586 |
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| DiO | 475 | 500 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
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| evoglow-Bs1 | 449 | 496 |
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| FITC (Fluorescein) | 495 | 519 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FM 1-43 | 473 | 579 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| Lucifer yellow | 428 | 544 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mTFP1 | 462 | 492 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Pro-Q Emerald | 289 | 530 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 45 | 451 | 485 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
|
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| TagCFP | 458 | 480 |
|
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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| YO-PRO-1 | 491 | 506 |
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| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-466/40-25
FL-004477 / 0
- Tavg > 93% 446 – 486 nm
- Center Wavelength of 446 nm and GMBW of 40 nm
- FWHM of 45.3 nm
FF03-525/50-25
FL-004656 / 0
- Tavg > 93% 500 – 550 nm
- Center Wavelength of 525 nm and GMBW of 50 nm
- FWHM of 55.9 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LP03-532RE-25
FL-009094