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BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

Semrock Part Number: GFP-4050B-NQF-ZERO

IDEX Part Number: S-001545  /

Semrock

This filter set is optimized to be the brightest filter set available for measuring GFP and its variants.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,573.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-466/40-25

FL-004477 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 446 – 486 nm
  • Center Wavelength of 446 nm and GMBW of 40 nm
  • FWHM of 45.3 nm
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FF03-525/50-25

FL-004656 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 500 – 550 nm
  • Center Wavelength of 525 nm and GMBW of 50 nm
  • FWHM of 55.9 nm
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FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBO™-1 461 484
Calcein 494 514
Cerulean 434 473
CFP (ECFP) 433 475
CFP2 490 510
Cy2™ 492 507
CyQuant Cell Proliferation Assay 502 523
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiA 457 586
DiO 475 500
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
Lucifer yellow 428 544
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
mHoneyDew 478 562
MitoTracker™ Green 490 512
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
PicoGreen® 502 522
PKH67 488 500
Pro-Q Emerald 289 530
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine Green 497 523
Rhodol Green 497 524
sgGFP™ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYTO 13 448 506
SYTO 16 489 520
SYTO 45 451 485
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagCFP 458 480
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

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FF01-466/40-25

FL-004477 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 446 – 486 nm
  • Center Wavelength of 446 nm and GMBW of 40 nm
  • FWHM of 45.3 nm
Compare Compare
More Info
Compare Compare

FF03-525/50-25

FL-004656 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 500 – 550 nm
  • Center Wavelength of 525 nm and GMBW of 50 nm
  • FWHM of 55.9 nm
Compare Compare
More Info
Compare Compare

FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence
Compare Compare
More Info
Compare Compare
Hide Show
{{compareProduct.AlternativeText}}
{{compareProduct.AlternativeText}}

/ 

1 of 1

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)

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