This filter set is optimized to be simultaneously a bright and high signal-to-noise ratio (contrast) for measuring GFP and its variants, allowing for no need to exchange filter sets for different GFP experiments.
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Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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Acridine Orange (+DNA) | 502 | 526 |
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Acridine Yellow | 470 | 550 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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AmCyan1 | 458 | 489 |
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ATTO 465 | 453 | 507 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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BCECF (pH 5.5) | 481 | 518 |
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BCECF (pH 9.0) | 502 | 528 |
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BOBOâ„¢-1 | 461 | 484 |
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BODIPY FL | 505 | 512 |
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Calcein | 494 | 514 |
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CFP (ECFP) | 433 | 475 |
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CFP2 | 490 | 510 |
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Cy2â„¢ | 492 | 507 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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Dendra2 (Green) | 491 | 507 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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ECFP | 435 | 475 |
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ecliptic pHluorin pH5.5 | 473 | 507 |
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Emerald | 491 | 511 |
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evoglow-Bs1 | 449 | 496 |
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FITC (Fluorescein) | 495 | 519 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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GFP (EGFP) | 489 | 511 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HiLyte Fluorâ„¢ 488 | 497 | 526 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LysoSensor Green | 448 | 502 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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mHoneyDew | 478 | 562 |
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MitoTrackerâ„¢ Green | 490 | 512 |
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mTFP1 | 462 | 492 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Oregon Greenâ„¢ 488 | 498 | 526 |
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PicoGreen® | 502 | 522 |
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PKH67 | 488 | 500 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine Green | 497 | 523 |
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Rhodol Green | 497 | 524 |
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sgGFPâ„¢ (super glow GFP) | 472 | 506 |
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Sodium Green | 507 | 532 |
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SpectrumGreen | 497 | 538 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYTO 11 | 506 | 525 |
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SYTO 13 | 448 | 506 |
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SYTO 16 | 489 | 520 |
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SYTO 45 | 451 | 485 |
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SYTO 9 | 483 | 500 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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TagCFP | 458 | 480 |
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TOTO-1 | 514 | 531 |
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TurboGFP | 482 | 503 |
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Vybrant DyeCycle Green | 506 | 534 |
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wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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YO-PRO-1 | 491 | 506 |
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YOYO-1 | 491 | 508 |
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Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
FL-009655