BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

Semrock Part Number: GFP-3035D-000

IDEX Part Number: S-000790  /

Semrock

This filter set is optimized to be simultaneously a bright and high signal-to-noise ratio (contrast) for measuring GFP and its variants, allowing for no need to exchange filter sets for different GFP experiments.

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  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,055.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-520/35-25

FL-004484

Each
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FL-004484

FF02-472/30-25

FL-004430

Each
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FL-004430

FF495-Di03-25x36

FL-007145

Each
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-007145

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

GFP-3035D-NQF

S-000266 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

GFP-3035D-NTE

S-000267 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

GFP-3035D-OMF

S-000270 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

GFP-3035D-ZHE

S-001077 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

GFP-3035D-OFF

S-000269 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

GFP-3035D-000-ZERO

S-000791 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

GFP-3035D-NQF-ZERO

S-001532 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series