This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|