This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,973.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-510/84-25
FL-004380 / 0
- Tavg > 93% 468 – 552 nm
- Center Wavelength of 510 nm and GMBW of 84 nm
- FWHM of 90 nm
FF01-340/26-25
FL-004440 / 0
- Tavg > 75% 327 – 353 nm
- Center Wavelength of 340 nm and GMBW of 26 nm
- FWHM of 30.2 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Fura-2 (calcium bound) | 336 | 505 |
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| Fura-2 (calcium free) | 366 | 515 |
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| LysoSensor Blue | 374 | 424 |
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| LysoSensor Yellow | 0 | 0 |
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total selected
Clear selection
Select all 4 products from all pages.
FF01-510/84-25
FL-004380 / 0
- Tavg > 93% 468 – 552 nm
- Center Wavelength of 510 nm and GMBW of 84 nm
- FWHM of 90 nm
FF01-340/26-25
FL-004440 / 0
- Tavg > 75% 327 – 353 nm
- Center Wavelength of 340 nm and GMBW of 26 nm
- FWHM of 30.2 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).