BrightLine® single-band multi-exciter filter set, optimized for FURA2 and other like fluorophores

Semrock Part Number: FURA2-C-NTE-ZERO

IDEX Part Number: S-000248  /

Semrock

This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.

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  • 1,973.00 USD Each
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FF01-510/84-25

FL-004380 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 468 – 552 nm
  • Center Wavelength of 510 nm and GMBW of 84 nm
  • FWHM of 90 nm

FF01-340/26-25

FL-004440 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 327 – 353 nm
  • Center Wavelength of 340 nm and GMBW of 26 nm
  • FWHM of 30.2 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Fura-2 (calcium bound) 336 505
Fura-2 (calcium free) 366 515
LysoSensor Blue 374 424
LysoSensor Yellow 0 0

Related Products

FF01-510/84-25

FL-004380 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 468 – 552 nm
  • Center Wavelength of 510 nm and GMBW of 84 nm
  • FWHM of 90 nm

FF01-340/26-25

FL-004440 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 327 – 353 nm
  • Center Wavelength of 340 nm and GMBW of 26 nm
  • FWHM of 30.2 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).