BrightLine® single-band multi-exciter filter set, optimized for FURA2 and other like fluorophores

Semrock Part Number: FURA2-C-NTE

IDEX Part Number: S-000247  /

Semrock

This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.

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  • Price Not Available.
  • 1,864.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-510/84-25

FL-004380 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 468 – 552 nm
  • Center Wavelength of 510 nm and GMBW of 84 nm
  • FWHM of 90 nm

FF01-340/26-25

FL-004440 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 327 – 353 nm
  • Center Wavelength of 340 nm and GMBW of 26 nm
  • FWHM of 30.2 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Fura-2 (calcium bound) 336 505
Fura-2 (calcium free) 366 515
LysoSensor Blue 374 424
LysoSensor Yellow 0 0

Related Products

FF01-510/84-25

FL-004380 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 468 – 552 nm
  • Center Wavelength of 510 nm and GMBW of 84 nm
  • FWHM of 90 nm

FF01-340/26-25

FL-004440 / 0

Out of Stock Backordered In Stock
  • Tavg > 75% 327 – 353 nm
  • Center Wavelength of 340 nm and GMBW of 26 nm
  • FWHM of 30.2 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)