BrightLine® single-band multi-exciter filter set, optimized for FURA2 and other like fluorophores

Semrock Part Number: FURA2-C-000

IDEX Part Number: S-000774  /

Semrock

This four-filter BrightLine fluorescence set is specially designed to achieve high-performance fast ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide superb balance of free and saturated Ca2+ signals, while minimizing the crosstalk and noise, resulting in the most accurate ratiometric measurements. The high signal throughput of the filter set permits reliable Fura-2 (Ca2+) measurements, even when using light sources of relatively limited ultraviolet output. Independent laboratories have measured this set to have 2-4X the free and saturated signals as compared with filters from the leading competitor.

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  • Price Not Available.
  • 1,469.55 USD Each
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We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components

FF01-340/26-25

FL-004440

Each
  • Tavg > 75% 327 – 353 nm
  • Center Wavelength of 340 nm and GMBW of 26 nm
  • FWHM of 30.2 nm

FL-004440

FF01-387/11-25

FL-004663

Each
  • Tavg > 90% 382 – 393 nm
  • Center Wavelength of 387.5 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FL-004663

FF01-510/84-25

FL-004380

Each
  • Tavg > 93% 468 – 552 nm
  • Center Wavelength of 510 nm and GMBW of 84 nm
  • FWHM of 90 nm

FL-004380

FF409-Di03-25x36

FL-007109

Each
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FL-007109

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMakerâ„¢ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

FURA2-C-U03

S-000778 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FURA2-C-U03-ZERO

S-000779 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FURA2-C-NQF

S-000246 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FURA2-C-NTE

S-000247 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

FURA2-C-OMF

S-000250 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

FURA2-C-ZHE

S-000252 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

FURA2-C-OFF

S-000249 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

FURA2-C

S-000775 / 0

Out of Stock Backordered In Stock

FURA2-C-NQF-ZERO

S-001516 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FURA2-C-NTE-ZERO

S-000248 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Fura-2 (calcium bound) 336 505
Fura-2 (calcium free) 366 515
LysoSensor Blue 374 424
LysoSensor Yellow 0 0

Specifications

FURA2-C-U03

S-000778 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FURA2-C-U03-ZERO

S-000779 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

FURA2-C-NQF

S-000246 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FURA2-C-NTE

S-000247 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

FURA2-C-OMF

S-000250 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

FURA2-C-ZHE

S-000252 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

FURA2-C-OFF

S-000249 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

FURA2-C

S-000775 / 0

Out of Stock Backordered In Stock

FURA2-C-NQF-ZERO

S-001516 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

FURA2-C-NTE-ZERO

S-000248 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.