BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

Semrock Part Number: FRET-GFP/RFP-D-NTE-ZERO

IDEX Part Number: S-000243  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field micro

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  • Price Not Available.
  • 1,948.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF02-472/30-25

FL-004430 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 488 499 520
Cy2™ 492 507
Cy3™ 554 566
DsRed 559 583
dTomato 556 582
FITC (Fluorescein) 495 519
GFP (EGFP) 489 511
mCherry 587 610
mRFP1 584 700
mStrawberry 574 596
TRITC (Tetramethylrhodamine) 552 578

Related Products

FF02-472/30-25

FL-004430 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)