This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field micro
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,839.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-472/30-25
FL-004430 / 0
- Tavg > 93% 457.5 – 487.5 nm
- Center Wavelength of 472 nm and GMBW of 30 nm
- FWHM of 34.6 nm
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
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Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Alexa Fluor® 488 | 499 | 520 |
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| Cy2™ | 492 | 507 |
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| Cy3™ | 554 | 566 |
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| DsRed | 559 | 583 |
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| dTomato | 556 | 582 |
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| FITC (Fluorescein) | 495 | 519 |
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| GFP (EGFP) | 489 | 511 |
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| mCherry | 587 | 610 |
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| mRFP1 | 584 | 700 |
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| mStrawberry | 574 | 596 |
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
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total selected
Clear selection
Select all 4 products from all pages.
FF02-472/30-25
FL-004430 / 0
- Tavg > 93% 457.5 – 487.5 nm
- Center Wavelength of 472 nm and GMBW of 30 nm
- FWHM of 34.6 nm
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF495-Di03-25x36
FL-007145 / 0
- Dichroic Beamsplitter with edge at 495 nm
- Ravg > 98% 350 – 487.5 nm
- Tavg > 93% 502.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description | |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters | |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters | |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD | |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? |
|