BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

Semrock Part Number: FRET-GFP/RFP-D-000-ZERO

IDEX Part Number: S-000773  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field micro

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  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,539.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-520/35

FL-004419

Each

FL-004419

FF02-472/30-25

FL-004430

Each
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FL-004430

FF02-641/75

FL-004437

Each

FL-004437

FF495-Di03

FL-007146

Each

FL-007146

FF02-472/30-25

FL-004430 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 488 499 520
Cy2™ 492 507
Cy3™ 554 566
DsRed 559 583
dTomato 556 582
FITC (Fluorescein) 495 519
GFP (EGFP) 489 511
mCherry 587 610
mRFP1 584 700
mStrawberry 574 596
TRITC (Tetramethylrhodamine) 552 578

Related Products

FF02-472/30-25

FL-004430 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 457.5 – 487.5 nm
  • Center Wavelength of 472 nm and GMBW of 30 nm
  • FWHM of 34.6 nm

FF02-641/75-25

FL-004436 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 604 – 679 nm
  • Center Wavelength of 641.5 nm and GMBW of 75 nm
  • FWHM of 81.7 nm

FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF495-Di03-25x36

FL-007145 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 495 nm
  • Ravg > 98% 350 – 487.5 nm
  • Tavg > 93% 502.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence