BrightLine® FRET filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Part Number: FRET-Cy3/Cy5-A-OFF

IDEX Part Number: S-001503  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

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  • 1,889.00 USD Each
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FF01-531/40-25

FL-004550 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FF01-593/40-25

FL-004557 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661 – 690 nm
  • Center Wavelength of 675.5 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Cy3™ 554 566
Cy5™ 649 666

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FF01-531/40-25

FL-004550 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FF01-593/40-25

FL-004557 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661 – 690 nm
  • Center Wavelength of 675.5 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence