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BrightLine® FRET filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Part Number: FRET-Cy3/Cy5-A-NTE

IDEX Part Number: S-001501  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,839.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-531/40-25

FL-004550

Each
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FL-004550

FF01-593/40-25

FL-004557

Each
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FL-004557

FF01-676/29-25

FL-004567

Each
  • Tavg > 90% 661.5 – 690.5 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FL-004567

FF562-Di03-25x36

FL-007122

Each
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-007122

TE 2000 Fluorescence Filter Holder for Nikon Microscopes

NTE

Each
Compatible microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

NTE

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Cy3™ 554 566
Cy5™ 649 666

NTE Holder/Cube

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)

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