BrightLine® FRET filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Part Number: FRET-Cy3/Cy5-A-LDMP-ZERO

IDEX Part Number: S-001498  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

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  • Price Not Available.
  • 1,998.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-531/40-25

FL-004550 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FF01-593/40-25

FL-004557 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661.5 – 690.5 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Cy3™ 554 566
Cy5™ 649 666

Related Products

FF01-531/40-25

FL-004550 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FF01-593/40-25

FL-004557 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661.5 – 690.5 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm