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BrightLine® FRET filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Part Number: FRET-Cy3/Cy5-A-000-ZERO

IDEX Part Number: S-002604  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for green donor fluorescence and red acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,539.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-531/40-25

FL-004550

Each
  • Tavg > 93% 511 – 551 nm
  • Center Wavelength of 531 nm and GMBW of 40 nm
  • FWHM of 45.8 nm

FL-004550

FF01-593/40-25

FL-004557

Each
  • Tavg > 93% 573 – 613 nm
  • Center Wavelength of 593 nm and GMBW of 40 nm
  • FWHM of 45.7 nm

FL-004557

FF01-676/29-25

FL-004567

Each
  • Tavg > 90% 661.5 – 690.5 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FL-004567

FF562-Di03

FL-007169

Each

FL-007169

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Cy3™ 554 566
Cy5™ 649 666

Fluorophore

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