BrightLine® FRET filter set, optimized for Cyan & Yellow Fluorescent Protein and other like fluorophores

Semrock Part Number: FRET-CFP/YFP-C-OMF

IDEX Part Number: S-001492  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for cyan donor fluorescence and yellow acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

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  • 1,889.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
AmCyan1 458 489
CFP (ECFP) 433 475
Venus 516 528
YFP (yellow GFP) 513 530

Related Products

FF01-483/32-25

FL-004598 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 467 – 499 nm
  • Center Wavelength of 483 nm and GMBW of 32 nm
  • FWHM of 38.2 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

FF02-438/24-25

FL-004649 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 426 – 450 nm
  • Center Wavelength of 438 nm and GMBW of 24 nm
  • FWHM of 29.5 nm

FF458-Di02-25x36

FL-007114 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 458 nm
  • Ravg > 98% 350 – 450 nm
  • Tavg > 93% 467 – 950 nm
  •  Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).