This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.
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FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Alexa Fluor® 350 | 343 | 441 |
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| Alexa Fluor® 488 | 499 | 520 |
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| BFP (EBFP) | 380 | 440 |
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| Cy2™ | 492 | 507 |
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| DAPI | 359 | 461 |
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| FITC (Fluorescein) | 495 | 519 |
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| GFP (EGFP) | 489 | 511 |
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total selected
Clear selection
Select all 4 products from all pages.
FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).