BrightLine® FRET filter set, optimized for Blue & Green Fluorescent Protein and other like fluorophores

Semrock Part Number: FRET-BFP/GFP-B-NQF

IDEX Part Number: S-001474  /

Semrock

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

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  • Price Not Available.
  • 1,839.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF02-447/60-25

FL-004651 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 417 – 477 nm
  • Center Wavelength of 447 nm and GMBW of 60 nm
  • FWHM of 65.3 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Alexa Fluor® 350 343 441
Alexa Fluor® 488 499 520
BFP (EBFP) 380 440
Cy2™ 492 507
DAPI 359 461
FITC (Fluorescein) 495 519
GFP (EGFP) 489 511

Related Products

FF01-520/35-25

FL-004484 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 537.5 nm
  • Center Wavelength of 520 nm and GMBW of 35 nm
  • FWHM of 40.7 nm

FF02-447/60-25

FL-004651 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 417 – 477 nm
  • Center Wavelength of 447 nm and GMBW of 60 nm
  • FWHM of 65.3 nm

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).