This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,928.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 381.5 – 392.5 nm
- Center Wavelength of 387 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Alexa Fluor® 350 | 343 | 441 |
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| Alexa Fluor® 488 | 499 | 520 |
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| BFP (EBFP) | 380 | 440 |
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| Cy2™ | 492 | 507 |
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| DAPI | 359 | 461 |
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| FITC (Fluorescein) | 495 | 519 |
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| GFP (EGFP) | 489 | 511 |
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total selected
Clear selection
Select all 4 products from all pages.
FF01-520/35-25
FL-004484 / 0
- Tavg > 93% 502.5 – 537.5 nm
- Center Wavelength of 520 nm and GMBW of 35 nm
- FWHM of 40.7 nm
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 381.5 – 392.5 nm
- Center Wavelength of 387 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1