This 5-filter "Sedat" set is ideal for high-speed sequential imaging FITC and Texas Red with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is also very popular for imaging GFP and mCherry. It is comprised of two single-band exciters and two single-band emitters, along with a dual-band beamsplitter, and thus works best when used with both exciter and emitter filter wheels.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,349.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-586/20-25x5
FL-004496 / 0
- Tavg > 93% 576 – 596 nm
- Center Wavelength of 586 nm and GMBW of 20 nm
- FWHM of 25.9 nm
FF01-628/32-25
FL-004501 / 0
- Tavg > 93% 612 – 644 nm
- Center Wavelength of 628 nm and GMBW of 32 nm
- FWHM of 38.2 nm
FF01-536/40-25
FL-004682 / 0
- Tavg > 93% 516 – 556 nm
- Center Wavelength of 536 nm and GMBW of 40 nm
- FWHM of 45.3 nm
FF505/606-Di01-25x36
FL-006825 / 0
- Dichroic Beamsplitter with edges at 505 nm & 606 nm
- Ravg > 95% 458 – 499 nm & Ravg > 95% 570 – 600 nm
- Tavg > 90% 509 – 541 nm & Tavg > 90% 612 – 647 nm
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 6-carboxyrhodamine 6G | 525 | 555 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 514 | 518 | 543 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 590 | 594 | 624 |
|
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| ATTO 594 | 601 | 624 |
|
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| ATTO 610 | 614 | 634 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY 630/650-X | 625 | 641 |
|
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| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| DiA | 457 | 586 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 594 | 593 | 618 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
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| Emerald | 491 | 511 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
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| Fluo-3 | 506 | 527 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| Hypericin | 600 | 603 |
|
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
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| mApple | 566 | 594 |
|
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| mCherry | 587 | 610 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| MitoTracker™ Red | 578 | 598 |
|
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| mKate2 | 588 | 633 |
|
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| mPlum | 589 | 649 |
|
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| mRFP1 | 584 | 700 |
|
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| mStrawberry | 574 | 596 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Oregon Green™ 514 | 513 | 532 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumRed | 587 | 615 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 17 | 619 | 638 |
|
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| SYTO 59 | 622 | 644 |
|
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| SYTO 61 | 618 | 650 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red® | 592 | 614 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| TurboGFP | 482 | 503 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
|
| YO-PRO-1 | 491 | 506 |
|
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| YO-PRO-3 | 613 | 629 |
|
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| YOYO-1 | 491 | 508 |
|
|
| YOYO-3 | 611 | 631 |
|