BrightLine Basic long-pass filter set, optimized for FITC and other like fluorophores

Semrock Part Number: FITC-LP01-Clinical-OMF

IDEX Part Number: S-001457  /

Semrock

This long-pass filter set is optimized for all popular clinical tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect (IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurate identification in test after test. Because it is a long-pass set, for visual applications it offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, and red) fluorescence from counter-stains such as Rhodamine and Evans Blue. For our best-value bandpass filter set, try FITC-A-Basic.

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  • 1,404.00 USD Each
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FITC-Di01-Clin-25x36

FL-007186 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 500 nm
  • Ravg > 98% 461.5 – 489.5 nm
  • Tavg > 90% 519 – 700 nm
  •  Flatness / RWE: Standard Epi-fluorescence

FITC-Ex01-Clin-25

FL-007187 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 461 – 489 nm
  • Center Wavelength of 475 nm and GMBW of 28
  • FWHM of 32 nm

FITC-LP01-Clin-25

FL-007188 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 515 nm
  • Tavg > 90% 519 – 700 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)26 mm x 38 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Orange (+RNA) 460 650
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
Calcein 494 514
CFP2 490 510
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Red) 554 569
Di-8-ANEPPS 467 631
DiA 457 586
Dronpa 502 516
DsRed-Express 556 584
DY-505-Phalloidin 503 530
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JC-1 499 525
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Yellow HCK-123 488 565
mHoneyDew 478 562
MitoTracker™ Green 490 512
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-triglyceride 510 583
Oregon Green™ 488 498 526
PerCP 490 677
PicoGreen® 502 522
Propidium Iodide (PI) 305 617
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Rhodamine 110 497 519
Rhodamine Green 497 523
Rhodol Green 497 524
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYPRO Ruby protein gel stain 469 631
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TOTO-1 514 531
Vybrant DyeCycle Green 506 534
YO-PRO-1 491 506
YOYO-1 491 508

Related Products

FITC-Di01-Clin-25x36

FL-007186 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 500 nm
  • Ravg > 98% 461.5 – 489.5 nm
  • Tavg > 90% 519 – 700 nm
  •  Flatness / RWE: Standard Epi-fluorescence

FITC-Ex01-Clin-25

FL-007187 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 461 – 489 nm
  • Center Wavelength of 475 nm and GMBW of 28
  • FWHM of 32 nm

FITC-LP01-Clin-25

FL-007188 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 515 nm
  • Tavg > 90% 519 – 700 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)26 mm x 38 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.