This long-pass filter set is optimized for all popular clinical tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect (IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurate identification in test after test. Because it is a long-pass set, for visual applications it offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, and red) fluorescence from counter-stains such as Rhodamine and Evans Blue. For our best-value bandpass filter set, try FITC-A-Basic.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,354.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FITC-Di01-Clin-25x36
FL-007186 / 0
- Dichroic Beamsplitter with edge at 500 nm
- Ravg > 98% 461.5 – 489.5 nm
- Tavg > 90% 519 – 700 nm
- Flatness / RWE: Standard Epi-fluorescence
FITC-Ex01-Clin-25
FL-007187 / 0
- Tavg > 90% 461 – 489 nm
- Center Wavelength of 475 nm and GMBW of 28
- FWHM of 32 nm
FITC-LP01-Clin-25
FL-007188 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Orange (+RNA) | 460 | 650 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| Calcein | 494 | 514 |
|
|
| CFP2 | 490 | 510 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| Di-8-ANEPPS | 467 | 631 |
|
|
| DiA | 457 | 586 |
|
|
| Dronpa | 502 | 516 |
|
|
| DsRed-Express | 556 | 584 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| FM 4-64 | 505 | 725 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| JC-1 | 499 | 525 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| PerCP | 490 | 677 |
|
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| PicoGreen® | 502 | 522 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FITC-Di01-Clin-25x36
FL-007186 / 0
- Dichroic Beamsplitter with edge at 500 nm
- Ravg > 98% 461.5 – 489.5 nm
- Tavg > 90% 519 – 700 nm
- Flatness / RWE: Standard Epi-fluorescence
FITC-Ex01-Clin-25
FL-007187 / 0
- Tavg > 90% 461 – 489 nm
- Center Wavelength of 475 nm and GMBW of 28
- FWHM of 32 nm
FITC-LP01-Clin-25
FL-007188 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |