This long-pass filter set is optimized for all popular clinical tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect (IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurate identification in test after test. Because it is a long-pass set, for visual applications it offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, and red) fluorescence from counter-stains such as Rhodamine and Evans Blue. For our best-value bandpass filter set, try FITC-A-Basic.
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||21.8 mm||5.0 mm|
|Emitter (Diameter)||23.3 mm||5.0 mm|
|Dichroic (L × W)||22.0 mm × 29.0 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|