BrightLine Basic single-band filter set, optimized for FITC and other like fluorophores

Semrock Part Number: FITC-A-Basic-ZHE

IDEX Part Number: S-000236  /

Semrock

This filter set is ideal for use with FITC and other popular green fluorophores. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with similar contrast. Want options? For hard-coated yet even more economical FITC filter sets optimized specifically for clinical applications, including a long-pass filter set, see our FITC-LP01-Clinical set. For the highest performance, try our flagship BrightLine FITC-3540C set. Compare BrightLine Basic filters to soft-coated filter sets and our highest-performance BrightLine filter sets.

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  • 1,244.00 USD Each
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FF01-475/35-25

FL-004539 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 457.3 – 492.7 nm
  • Center Wavelength of 475 nm and GMBW of 35.4 nm
  • FWHM of 40.1 nm

FF01-530/43-25

FL-004549 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 508.7 – 551.4 nm
  • Center Wavelength of 530 nm and GMBW of 42.7 nm
  • FWHM of 48 nm

FF499-Di01-25x36

FL-007094 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 499 nm
  • Ravg > 90% 470 – 490 nm
  • Tavg > 90% 508 – 675 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBO™-1 461 484
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP (ECFP) 433 475
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiA 457 586
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mTFP1 462 492
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine Green 497 523
Rhodol Green 497 524
rsGFP (red shifted GFP, S65T) 498 516
sgGFP™ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 45 451 485
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagCFP 458 480
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

Related Products

FF01-475/35-25

FL-004539 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 457.3 – 492.7 nm
  • Center Wavelength of 475 nm and GMBW of 35.4 nm
  • FWHM of 40.1 nm

FF01-530/43-25

FL-004549 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 508.7 – 551.4 nm
  • Center Wavelength of 530 nm and GMBW of 42.7 nm
  • FWHM of 48 nm

FF499-Di01-25x36

FL-007094 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 499 nm
  • Ravg > 90% 470 – 490 nm
  • Tavg > 90% 508 – 675 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.