This filter set is ideal for use with FITC and other popular green fluorophores. This hard-coated filter set is the best value on the market, delivering higher brightness than the comparable premium soft-coated filter sets with similar contrast. Want options? For hard-coated yet even more economical FITC filter sets optimized specifically for clinical applications, including a long-pass filter set, see our FITC-LP01-Clinical set. For the highest performance, try our flagship BrightLine FITC-3540C set. Compare BrightLine Basic filters to soft-coated filter sets and our highest-performance BrightLine filter sets.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,354.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-475/35-25
FL-004539 / 0
- Tavg > 90% 457.3 – 492.7 nm
- Center Wavelength of 475 nm and GMBW of 35.4 nm
- FWHM of 40.1 nm
FF01-530/43-25
FL-004549 / 0
- Tavg > 90% 508.7 – 551.4 nm
- Center Wavelength of 530 nm and GMBW of 42.7 nm
- FWHM of 48 nm
FF499-Di01-25x36
FL-007094 / 0
- Dichroic Beamsplitter with edge at 499 nm
- Ravg > 90% 470 – 490 nm
- Tavg > 90% 508 – 675 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| ATTO 465 | 453 | 507 |
|
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| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| BODIPY FL-MeOH | 502 | 511 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
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| Dendra2 (Green) | 491 | 507 |
|
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| DiA | 457 | 586 |
|
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| DiO | 475 | 500 |
|
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| Dronpa | 502 | 516 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
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| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
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| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| mTFP1 | 462 | 492 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| ratiometric pHluorin pH5 | 500 | 509 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| rsGFP (red shifted GFP, S65T) | 498 | 516 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 45 | 451 | 485 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| TagCFP | 458 | 480 |
|
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| TOTO-1 | 514 | 531 |
|
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| TurboGFP | 482 | 503 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-475/35-25
FL-004539 / 0
- Tavg > 90% 457.3 – 492.7 nm
- Center Wavelength of 475 nm and GMBW of 35.4 nm
- FWHM of 40.1 nm
FF01-530/43-25
FL-004549 / 0
- Tavg > 90% 508.7 – 551.4 nm
- Center Wavelength of 530 nm and GMBW of 42.7 nm
- FWHM of 48 nm
FF499-Di01-25x36
FL-007094 / 0
- Dichroic Beamsplitter with edge at 499 nm
- Ravg > 90% 470 – 490 nm
- Tavg > 90% 508 – 675 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.