This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate), 5-FAM, and Alexa Fluor 488. It provides the highest brightness and is ideal for similar fluorophores that have narrower, more closely spaced excitation and emission peaks, like BODIPY.
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1,477.63 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-475/50-25
FL-004198 / 0
- Tavg > 93% 450 – 500 nm
- Center Wavelength of 475 nm and GMBW of 50 nm
- FWHM of 55.7 nm
FF01-540/50-25
FL-004307 / 0
- Tavg > 93% 515.5 – 565.5 nm
- Center Wavelength of 540.5 nm and GMBW of 50 nm
- FWHM of 55.6 nm
FF506-Di03-25x36
FL-007149 / 0
- Dichroic Beamsplitter with edge at 506 nm
- Ravg > 98% 350 – 500 nm
- Tavg > 93% 513 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Yellow | 470 | 550 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
|
| AmCyan1 | 458 | 489 |
|
|
| ATTO 465 | 453 | 507 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BODIPY FL | 505 | 512 |
|
|
| BODIPY FL-MeOH | 502 | 511 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| CFP2 | 490 | 510 |
|
|
| Cy2™ | 492 | 507 |
|
|
| CyQUANT GR-DNA | 502 | 523 |
|
|
| DAF-FM-NO | 495 | 519 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| DiA | 457 | 586 |
|
|
| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| DY-505-Phalloidin | 503 | 530 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluo-4 | 494 | 516 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| GFP (EGFP) | 489 | 511 |
|
|
| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
|
| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
|
| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| LysoTracker Green | 501 | 509 |
|
|
| LysoTracker Yellow HCK-123 | 488 | 565 |
|
|
| Magnesium Green | 507 | 531 |
|
|
| mHoneyDew | 478 | 562 |
|
|
| MitoTracker™ Green | 490 | 512 |
|
|
| mWasabi | 493 | 509 |
|
|
| NBD-X (MeOH) | 467 | 538 |
|
|
| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
|
| PicoGreen® | 502 | 522 |
|
|
| PKH67 | 488 | 500 |
|
|
| Pro-Q Emerald | 289 | 530 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| ratiometric pHluorin pH5 | 500 | 509 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
|
| Rhodamine Green | 497 | 523 |
|
|
| Rhodol Green | 497 | 524 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| Sodium Green | 507 | 532 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TagCFP | 458 | 480 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboGFP | 482 | 503 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-475/50-25
FL-004198 / 0
- Tavg > 93% 450 – 500 nm
- Center Wavelength of 475 nm and GMBW of 50 nm
- FWHM of 55.7 nm
FF01-540/50-25
FL-004307 / 0
- Tavg > 93% 515.5 – 565.5 nm
- Center Wavelength of 540.5 nm and GMBW of 50 nm
- FWHM of 55.6 nm
FF506-Di03-25x36
FL-007149 / 0
- Dichroic Beamsplitter with edge at 506 nm
- Ravg > 98% 350 – 500 nm
- Tavg > 93% 513 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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Di03-R532-t3-25x36
FL-006803