BrightLine® single-band filter set, optimized for FITC and other like fluorophores

Semrock Part Number: FITC-5050A-ZHE-ZERO

IDEX Part Number: S-000233  /

Semrock

This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate), 5-FAM, and Alexa Fluor 488. It provides the highest brightness and is ideal for similar fluorophores that have narrower, more closely spaced excitation and emission peaks, like BODIPY.

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  • 1,463.00 USD Each
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FF02-475/50-25

FL-004198 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 450 – 500 nm
  • Center Wavelength of 475 nm and GMBW of 50 nm
  • FWHM of 55.7 nm

FF01-540/50-25

FL-004307 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 515.5 – 565.5 nm
  • Center Wavelength of 540.5 nm and GMBW of 50 nm
  • FWHM of 55.6 nm

FF506-Di03-25x36

FL-007149 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 506 nm
  • Ravg > 98% 350 – 500 nm
  • Tavg > 93% 513 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
Acridine Orange (+DNA) 502 526
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 465 453 507
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBOâ„¢-1 461 484
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
Cerulean 434 473
CFP (ECFP) 433 475
CFP2 490 510
Cy2â„¢ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiA 457 586
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluorâ„¢ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
Lucifer yellow 428 544
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTrackerâ„¢ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Greenâ„¢ 488 498 526
Oregon Greenâ„¢ 514 513 532
PicoGreen® 502 522
PKH67 488 500
Pro-Q Emerald 289 530
Qdot® 525 Nanocrystals 300 525
Qdot® 565 Nanocrystals 300 564
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
sgGFPâ„¢ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 45 451 485
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TagCFP 458 480
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

Related Products

FF02-475/50-25

FL-004198 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 450 – 500 nm
  • Center Wavelength of 475 nm and GMBW of 50 nm
  • FWHM of 55.7 nm

FF01-540/50-25

FL-004307 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 515.5 – 565.5 nm
  • Center Wavelength of 540.5 nm and GMBW of 50 nm
  • FWHM of 55.6 nm

FF506-Di03-25x36

FL-007149 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 506 nm
  • Ravg > 98% 350 – 500 nm
  • Tavg > 93% 513 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence