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BrightLine® single-band filter set, optimized for FITC and other like fluorophores

Semrock Part Number: FITC-3540C-000

IDEX Part Number: S-000752  /

Semrock

This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate), 5-FAM, and Alexa Fluor 488. It provides the both high brightness and signal-to-noise ratio. It is also ideal for similar fluorophores that have narrower, more closely spaced excitation and emission peaks, like BODIPY.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,055.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
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Set Components

FF01-482/35-25

FL-004447

Each
  • Tavg > 93% 464.5 – 499.5 nm
  • Center Wavelength of 482 nm and GMBW of 35 nm
  • FWHM of 39.1 nm

FL-004447

FF01-536/40-25

FL-004682

Each
  • Tavg > 93% 516 – 556 nm
  • Center Wavelength of 536 nm and GMBW of 40 nm
  • FWHM of 45.3 nm

FL-004682

FF506-Di03-25x36

FL-007149

Each
  • Dichroic Beamsplitter with edge at 506 nm
  • Ravg > 98% 350 – 500 nm
  • Tavg > 93% 513 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

FL-007149

Optical Specifications Value
Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
General Filter Specifications Value
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Physical Filter Specifications   Value
Scratch-Dig 60-40
Dichroic Orientation Reflective coating side should face toward light source.

FITC-3540C-U03

S-000759 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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FITC-3540C-U03-ZERO

S-000760 / 0

Out of Stock Backordered In Stock
This set is delivered unmounted and fits Olympus OFF cubes which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
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FITC-3540C-NQF

S-000219 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

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FITC-3540C-NTE

S-000220 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

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FITC-3540C-OMF

S-000222 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

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FITC-3540C-ZHE

S-000224 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

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FITC-3540C-OFF

S-001117 / 0

Out of Stock Backordered In Stock

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

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FITC-3540C-000-ZERO

S-000754 / 0

Out of Stock Backordered In Stock

Standard size -ZERO pixel set

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