This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate), 5-FAM, and Alexa Fluor 488. It provides the highest signal-to-noise ratio (contrast) and is ideal for similar fluorophores that have narrower, more closely spaced excitation and emission peaks, like BODIPY.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,194.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
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FL-004083 |
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FF02-485/20-25FL-004432
Each
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FL-004432 |
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FL-007095 |
total selected
Clear selection
Select all 3 products from all pages.
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-524/24-25
FL-004545 / 0
- Tavg > 93% 512.5 – 536.5 nm
- Center Wavelength of 524.5 nm and GMBW of 24 nm
- FWHM of 29.6 nm
FF506-Di03-25x36
FL-007149 / 0
- Dichroic Beamsplitter with edge at 506 nm
- Ravg > 98% 350 – 500 nm
- Tavg > 93% 513 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| AmCyan1 | 458 | 489 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| ratiometric pHluorin pH5 | 500 | 509 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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| YFP (yellow GFP) | 513 | 530 |
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| YO-PRO-1 | 491 | 506 |
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| YOYO-1 | 491 | 508 |
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total selected
Clear selection
Select all 3 products from all pages.
FF02-485/20-25
FL-004432 / 0
- Tavg > 93% 475 – 495 nm
- Center Wavelength of 485 nm and GMBW of 20 nm
- FWHM of 24.9 nm
FF01-524/24-25
FL-004545 / 0
- Tavg > 93% 512.5 – 536.5 nm
- Center Wavelength of 524.5 nm and GMBW of 24 nm
- FWHM of 29.6 nm
FF506-Di03-25x36
FL-007149 / 0
- Dichroic Beamsplitter with edge at 506 nm
- Ravg > 98% 350 – 500 nm
- Tavg > 93% 513 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence