Optical Specifications	Value
Transmission Band 1	Tavg > 90% 400 – 820 nm
Transmission Band 2	Tavg > 80% 400 – 420 nm (Rolling Window: 10 nm)
Transmission Band 3	Tavg > 90% 420 – 820 nm (Rolling Window: 10 nm)
Reflection Band 1	Ravg > 94% 1200 – 1350 nm
Reflection Band 1 (p-pol)	Ravg > 90% 1200 – 1350 nm
Reflection Band 1 (s-pol)	Ravg > 98% 1200 – 1350 nm
Reflection Band 2	Ravg > 94% 1600 – 1870 nm
Reflection Band 2 (p-pol)	Ravg > 90% 1600 – 1870 nm
Reflection Band 2 (s-pol)	Ravg > 98% 1600 – 1870 nm
Edge Wavelength 1	835 nm
Laser Wavelengths 1	1200 - 1350 nm
Laser Wavelengths 2	1600 - 1870 nm
General Filter Specifications	Value
Angle of Incidence	45 ± 1.5 degrees
Cone Half-angle	2 degrees
Optical Damage Rating	1 J/cm² @ 532 nm (10 ns pulse width)
Flatness / RWE Classification	Super-resolution / TIRF
Maximum Reflected Laser Beam Diameter	6 mm
Nominal Flatness	< 1.4λ P-V per inch @ 632.8 nm
Reflected Wavefront Error	< 2.5λ P-V RWE @ 632.8 nm
Steepness	Standard
Group Delay Dispersion 1	± 1500 fs² over 1200 - 1350 nm refl band for S-Pol and P-Pol
Group Delay Dispersion 2	± 750 fs² over 1600 - 1870 nm refl band for S-Pol and P-Pol
Physical Filter Specifications	Value
Transverse Dimensions (L x W)	25.2 mm x 35.6 mm
Transverse Tolerance	± 0.1 mm
Clear Aperture	≥ 80% (elliptical)
Scratch-Dig	60-40
Substrate Type	Fused Silica
Filter Thickness (unmounted)	1.05 mm
Filter Thickness Tolerance (unmounted)	± 0.05 mm
Orientation	Reflective surface marked with laser dot - Orient in direction of incoming light

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic Beamsplitters	Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage Threshold	Provides information for calculating laser damage threshold for pulsed and continuous wave lasers
Multiband Filter Set Terminology	Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence Microscopes	Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

Topic	Description
Bringing Super-resolution to Fluorescence Microscopy	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF Optics	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

Topic	Description
TIRF (Total Internal Reflection Fluorescence)	An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal Microscopy	An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based Instrumentation	An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

835 nm BrightLine® multiphoton 3P short-pass super-resolution / TIRF dichroic beamsplitter

Specifications