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835 nm BrightLine® multiphoton 3P short-pass super-resolution / TIRF dichroic beamsplitter

Semrock Part Number: FF835-SDi01-t1-25x36

IDEX Part Number: FL-413699  /

Semrock
Innovative short pass dichroic beamsplitter optimized for 3-photon laser excitation in a standard epi-fluorescence microscope configuration with reflected excitation and transmitted emission.

The 2-photon (Red) and 3-photon (Green & Red) excitation regions are ideal for femtosecond pulsed lasers such as Ti:Sapphire, OPO or OPA coupled, and neodymium- and ytterbium-doped lasers, enabling deep tissue imaging with improved contrast. This multiphoton dichroic has low reflected GDD that helps minimize temporal pulse broadening of reflected 1300 nm and 1700 nm laser pulses, and very high and flat reflection and transmission bands.

Semrock's Super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and super-resolution techniques such as TIRF, PALM, STORM, structured illumination, STED, and multiphoton imaging.

2.5λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 6 mm in diameter while minimizing RWE
λ/3 P-V RWE on 3 mm, optimized for reflecting laser beams up to 16.7 mm in diameter while minimizing RWE
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  • Price Not Available.
  • 1,197.70 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Optical Specifications Value
Transmission Band 1 Tavg > 90% 400 – 820 nm
Transmission Band 2 Tavg > 80% 400 – 420 nm (Rolling Window: 10 nm)
Transmission Band 3 Tavg > 90% 420 – 820 nm (Rolling Window: 10 nm)
Reflection Band 1 Ravg > 94% 1200 – 1350 nm
Reflection Band 1 (p-pol) Ravg > 90% 1200 – 1350 nm
Reflection Band 1 (s-pol) Ravg > 98% 1200 – 1350 nm
Reflection Band 2 Ravg > 94% 1600 – 1870 nm
Reflection Band 2 (p-pol) Ravg > 90% 1600 – 1870 nm
Reflection Band 2 (s-pol) Ravg > 98% 1600 – 1870 nm
Edge Wavelength 1 835 nm
Laser Wavelengths 1 1200 - 1350 nm
Laser Wavelengths 2 1600 - 1870 nm
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating 1 J/cm² @ 532 nm (10 ns pulse width)
Flatness / RWE Classification   Super-resolution / TIRF
Maximum Reflected Laser Beam Diameter 6 mm
Nominal Flatness < 1.4λ P-V per inch @ 632.8 nm
Reflected Wavefront Error < 2.5λ P-V RWE @ 632.8 nm
Steepness Standard
Group Delay Dispersion 1 ± 1500 fs² over 1200 - 1350 nm refl band for S-Pol and P-Pol
Group Delay Dispersion 2 ± 750 fs² over 1600 - 1870 nm refl band for S-Pol and P-Pol
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Substrate Type Fused Silica
Filter Thickness (unmounted) 1.05 mm
Filter Thickness Tolerance (unmounted) ± 0.05 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light

FF700-SDi01-25x36

FL-007162 / 0

Out of Stock Backordered In Stock
  • Multiphoton Dichroic Beamsplitter with edge at 700 nm
  • Ravg > 90% 725 – 1300 nm
  • Ravg, s-pol > 95% 720 – 1300 nm
  • Ravg, p-pol > 85% 730 – 1300 nm
  • Tavg > 90% 360 - 675 nm

 

 

 

FF880-SDi01-t1-25x36

FL-418430 / 0

Out of Stock Backordered In Stock
  • 2P Multiphoton Dichroic Beamsplitter with edge at 880 nm
  • Ravg, avg-pol > 94% 910 – 1400 nm
  • Tavg > 90% 400 – 850 nm
  •  Flatness / RWE: Super-resolution / TIRF

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Application
TopicDescription
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Specifications

FF700-SDi01-25x36

FL-007162 / 0

Out of Stock Backordered In Stock
  • Multiphoton Dichroic Beamsplitter with edge at 700 nm
  • Ravg > 90% 725 – 1300 nm
  • Ravg, s-pol > 95% 720 – 1300 nm
  • Ravg, p-pol > 85% 730 – 1300 nm
  • Tavg > 90% 360 - 675 nm

 

 

 

FF880-SDi01-t1-25x36

FL-418430 / 0

Out of Stock Backordered In Stock
  • 2P Multiphoton Dichroic Beamsplitter with edge at 880 nm
  • Ravg, avg-pol > 94% 910 – 1400 nm
  • Tavg > 90% 400 – 850 nm
  •  Flatness / RWE: Super-resolution / TIRF

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 1

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Application
TopicDescription
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.