738 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

Semrock Part Number: FF738-FDi01-t3-25x36

IDEX Part Number: FL-415920  /

Semrock
BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Common Fluorophore Pair to Split: Cy5.5/Cy7

FF738-FDi01-t1 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE
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  • Each
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  • Price Not Available.
  • 725.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 754 – 1200 nm
Reflection Band 1 Ravg > 95% 400 – 722 nm
Edge Wavelength 1 738 nm
Common Fluorophore Pair to Split Cy5.5/Cy7
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Reflected Wavefront Error < 0.2λ P-V RWE @ 632.8 nm
Steepness Standard
Flatness / RWE Classification   Image-splitting
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 3.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light, Reflective surface marked with part number - Orient in direction of incoming light

FF484-FDi01-25x36

FL-007022 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 950 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  • Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

FF509-FDi01-25x36

FL-007117 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 509 nm
  • Ravg > 95% 350 – 500 nm
  • Tavg > 93% 518.3 – 950 nm
  • Common Fluorophore Pair to Split: CFP/YFP
  • Flatness / RWE: Image-splitting

FF538-FDi01-25x36

FL-007120 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 538 nm
  • Ravg > 95% 350 – 528.4 nm
  • Tavg > 93% 547.7 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mOrange
  • Flatness / RWE: Image-splitting

FF560-FDi01-25x36

FL-007151 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 560 nm
  • Ravg > 95% 350 – 550 nm
  • Tavg > 93% 570.1 – 950 nm
  • Common Fluorophore Pair to Split: YFP/dTomato
  • Flatness / RWE: Image-splitting

FF580-FDi01-25x36

FL-007059 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 580 nm
  • Ravg > 95% 350 – 570 nm
  • Tavg > 93% 590.8 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mCherry
  •  Flatness / RWE: Image-splitting

FF640-FDi01-25x36

FL-006976 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 640 nm
  • Ravg > 95% 350 – 629.5 nm
  • Tavg > 93% 652 – 950 nm
  • Common Fluorophore Pair to Split: Cy3/Cy5
  •  Flatness / RWE: Image-splitting

FF662-FDi01-25x36

FL-007063 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 662 nm
  • Ravg > 95% 350 – 650 nm
  • Tavg > 93% 673.7 – 950 nm
  • Common Fluorophore Pair to Split: TxRed/Cy5
  • Flatness / RWE: Image-splitting