738 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

Semrock Part Number: FF738-FDi01-t3-25x36

IDEX Part Number: FL-415920  /

Semrock
BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Common Fluorophore Pair to Split: Cy5.5/Cy7

FF738-FDi01-t1 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE
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  • Price Not Available.
  • 732.25 USD Each
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 754 – 1200 nm
Reflection Band 1 Ravg > 95% 400 – 722 nm
Edge Wavelength 1 738 nm
Common Fluorophore Pair to Split Cy5.5/Cy7
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Flatness / RWE Classification   Image-splitting
Reflected Wavefront Error < 0.2λ P-V RWE @ 632.8 nm
Steepness Standard
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 3.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light, Reflective surface marked with part number - Orient in direction of incoming light

FF484-FDi01-25x36

FL-007022 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 950 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  • Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

FF509-FDi01-25x36

FL-007117 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 509 nm
  • Ravg > 95% 350 – 500 nm
  • Tavg > 93% 518.3 – 950 nm
  • Common Fluorophore Pair to Split: CFP/YFP
  • Flatness / RWE: Image-splitting

FF538-FDi01-25x36

FL-007120 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 538 nm
  • Ravg > 95% 350 – 528.4 nm
  • Tavg > 93% 547.7 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mOrange
  • Flatness / RWE: Image-splitting

FF560-FDi01-25x36

FL-007151 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 560 nm
  • Ravg > 95% 350 – 550 nm
  • Tavg > 93% 570.1 – 950 nm
  • Common Fluorophore Pair to Split: YFP/dTomato
  • Flatness / RWE: Image-splitting

FF580-FDi01-25x36

FL-007059 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 580 nm
  • Ravg > 95% 350 – 570 nm
  • Tavg > 93% 590.8 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mCherry
  •  Flatness / RWE: Image-splitting

FF640-FDi01-25x36

FL-006976 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 640 nm
  • Ravg > 95% 350 – 629 nm
  • Tavg > 93% 652 – 950 nm
  • Common Fluorophore Pair to Split: Cy3/Cy5
  •  Flatness / RWE: Image-splitting

FF662-FDi01-25x36

FL-007063 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 662 nm
  • Ravg > 95% 350 – 650 nm
  • Tavg > 93% 673.7 – 950 nm
  • Common Fluorophore Pair to Split: TxRed/Cy5
  • Flatness / RWE: Image-splitting

FF699-FDi01-t1-25x36

FL-415684 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 699 nm
  • Ravg > 95% 350 – 686 nm
  • Tavg > 93% 712 – 1200 nm
  • Common Fluorophore Pair to Split: iRFP670/iRFP720
  •  Flatness / RWE: Image-splitting

FF738-FDi01-t1-25x36

FL-415919 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 738 nm
  • Ravg > 95% 400 – 722 nm
  • Tavg > 93% 754 – 1200 nm
  • Common Fluorophore Pair to Split: Cy5.5/Cy7
  • Flatness / RWE: Image-splitting

FF484-FDi02-t3-25x36

FL-007053 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 1200 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  •  Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Measuring Light with Wavelengths & WavenumbersCompares units of wavelength to wavenumbers and provides formulas for calculating wavenumber shift
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Filter Types for Raman Spectroscopy ApplicationsCompares the performance of different combinations of LWP and SWP edge filters, notch and laser line filters for Raman applications
RazorEdge Filter LayoutsDescribes two different filter layouts that are commonly used in Raman applications
Journal Article
TopicDescription
Thin-Film Filters for Raman SpectroscopyTuran Erdogan, PhD, and Victor Mizrahi, PhD, Semrock Inc., Spectroscopy, December 2004
Describes the types and functions of filters commonly used in Raman spectroscopy
White Paper
TopicDescription
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Application
TopicDescription
Raman SpectroscopyAn overview of Raman spectroscopy, including solutions from Semrock and additional learning resources.

Specifications

FF484-FDi01-25x36

FL-007022 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 950 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  • Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

FF509-FDi01-25x36

FL-007117 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 509 nm
  • Ravg > 95% 350 – 500 nm
  • Tavg > 93% 518.3 – 950 nm
  • Common Fluorophore Pair to Split: CFP/YFP
  • Flatness / RWE: Image-splitting

FF538-FDi01-25x36

FL-007120 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 538 nm
  • Ravg > 95% 350 – 528.4 nm
  • Tavg > 93% 547.7 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mOrange
  • Flatness / RWE: Image-splitting

FF560-FDi01-25x36

FL-007151 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 560 nm
  • Ravg > 95% 350 – 550 nm
  • Tavg > 93% 570.1 – 950 nm
  • Common Fluorophore Pair to Split: YFP/dTomato
  • Flatness / RWE: Image-splitting

FF580-FDi01-25x36

FL-007059 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 580 nm
  • Ravg > 95% 350 – 570 nm
  • Tavg > 93% 590.8 – 950 nm
  • Common Fluorophore Pair to Split: GFP/mCherry
  •  Flatness / RWE: Image-splitting

FF640-FDi01-25x36

FL-006976 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 640 nm
  • Ravg > 95% 350 – 629 nm
  • Tavg > 93% 652 – 950 nm
  • Common Fluorophore Pair to Split: Cy3/Cy5
  •  Flatness / RWE: Image-splitting

FF662-FDi01-25x36

FL-007063 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 662 nm
  • Ravg > 95% 350 – 650 nm
  • Tavg > 93% 673.7 – 950 nm
  • Common Fluorophore Pair to Split: TxRed/Cy5
  • Flatness / RWE: Image-splitting

FF699-FDi01-t1-25x36

FL-415684 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 699 nm
  • Ravg > 95% 350 – 686 nm
  • Tavg > 93% 712 – 1200 nm
  • Common Fluorophore Pair to Split: iRFP670/iRFP720
  •  Flatness / RWE: Image-splitting

FF738-FDi01-t1-25x36

FL-415919 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 738 nm
  • Ravg > 95% 400 – 722 nm
  • Tavg > 93% 754 – 1200 nm
  • Common Fluorophore Pair to Split: Cy5.5/Cy7
  • Flatness / RWE: Image-splitting

FF484-FDi02-t3-25x36

FL-007053 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 1200 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  •  Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

1 of 2

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Measuring Light with Wavelengths & WavenumbersCompares units of wavelength to wavenumbers and provides formulas for calculating wavenumber shift
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Filter Types for Raman Spectroscopy ApplicationsCompares the performance of different combinations of LWP and SWP edge filters, notch and laser line filters for Raman applications
RazorEdge Filter LayoutsDescribes two different filter layouts that are commonly used in Raman applications
Journal Article
TopicDescription
Thin-Film Filters for Raman SpectroscopyTuran Erdogan, PhD, and Victor Mizrahi, PhD, Semrock Inc., Spectroscopy, December 2004
Describes the types and functions of filters commonly used in Raman spectroscopy
White Paper
TopicDescription
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Application
TopicDescription
Raman SpectroscopyAn overview of Raman spectroscopy, including solutions from Semrock and additional learning resources.