580 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

Semrock Part Number: FF580-FDi02-t3-25x36

IDEX Part Number: FL-007125  /

Semrock
BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Semrock's image-splitting dichroics for super-resolution microscopy deliver industry-leading λ/5 P-V RWE on 3 mm thick substrates for minimal focus shift and optical wavefront aberrations of the reflected imaging beam. These dichroics can be used with much larger diameter imaging beams up to 37 mm while minimizing RWE in custom size versions.

Common Fluorophore Pairs to Split: GFP/mCherry or FITC/TxRed

FF580-FDi01 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE
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  • Price Not Available.
  • 630.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 590.8 – 1200 nm
Reflection Band 1 Ravg > 95% 350 – 570 nm
Edge Wavelength 1 580 nm
Common Fluorophore Pair to Split GFP/mCherry or FITC/TxRed
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Reflected Wavefront Error < 0.2λ P-V RWE @ 632.8 nm
Steepness Standard
Flatness / RWE Classification   Image-splitting
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 3.0 mm
Substrate Thickness (3 mm, unmounted) 3.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Substrate Thickness Tolerance (3 mm, unmounted) ± 0.1 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light

FF484-FDi02-t3-25x36

FL-007053 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 484 nm
  • Ravg > 95% 350 – 475 nm
  • Tavg > 93% 492.3 – 1200 nm
  • Common Fluorophore Pair to Split: DAPI/FITC
  •  Flatness / RWE: Image-splitting

Custom sizing delivery confirmed ARO (sizing fee applies).

FF509-FDi02-t3-25x36

FL-006605 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 509 nm
  • Ravg > 95% 350 – 500 nm
  • Tavg > 93% 518.3 – 1200 nm
  • Common Fluorophore Pair to Split: CFP/YFP
  •  Flatness / RWE: Image-splitting

FF538-FDi02-t3-25x36

FL-006640 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 538 nm
  • Ravg > 95% 350 – 528.4 nm
  • Tavg > 93% 547.7 – 1200 nm
  • Common Fluorophore Pair to Split: GFP/mOrange
  •  Flatness / RWE: Image-splitting

FF560-FDi02-t3-25x36

FL-007057 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 560 nm
  • Ravg > 95% 350 – 550 nm
  • Tavg > 93% 570.1 – 1200 nm
  • Common Fluorophore Pair to Split: YFP/dTomato
  • Flatness / RWE: Image-splitting

FF580-FDi02-t3-25x36

FL-007125 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 580 nm
  • Ravg > 95% 350 – 570 nm
  • Tavg > 93% 590.8 – 1200 nm
  • Common Fluorophore Pair to Split: GFP/mCherry
  •  Flatness / RWE: Image-splitting

FF640-FDi02-t3-25x36

FL-006894 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 640 nm
  • Ravg > 95% 350 – 629 nm
  • Tavg > 93% 652 – 1200 nm
  • Common Fluorophore Pair to Split: Cy3/Cy5
  •  Flatness / RWE: Image-splitting

FF662-FDi02-t3-25x36

FL-006752 / 0

Out of Stock Backordered In Stock
  • Imaging Dichroic Beamsplitter with edge at 662 nm
  • Ravg > 95% 350 – 650 nm
  • Tavg > 93% 673.7 – 1200 nm
  • Common Fluorophore Pair to Split: TxRed/Cy5
  •  Flatness / RWE: Image-splitting