509 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for standard microscopy

Semrock Part Number: FF509-FDi01-25x36

IDEX Part Number: FL-007117  /

BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems. This dichroic beamsplitter is optimized for reflecting imaging beams up to 10 mm in diameter while minimizing RWE.

Common Fluorophore Pair to Split: CFP/YFP

Interested in an improved Flatness / RWE version? Consider Semrock's image-splitting dichoric beamsplitter for super-resolution microscopy:
λ/5 P-V RWE on 3mm FF509-FDi02-t3, optimized for reflecting imaging beams up to 37 mm in custom size versions.
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  • 390.00 USD Each
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 518.3 – 950 nm
Reflection Band 1 Ravg > 95% 350 – 500 nm
Edge Wavelength 1 509 nm
Common Fluorophore Pair to Split CFP/YFP
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Reflected Wavefront Error < 2λ P-V RWE @ 632.8 nm
Steepness Standard
Flatness / RWE Classification   Image-splitting
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 1.05 mm
Filter Thickness Tolerance (unmounted) ± 0.05 mm
Orientation Reflective surface marked with part number - Orient in direction of incoming light