421/491/567/659/776 nm BrightLine® penta-edge standard epi-fluorescence dichroic beamsplitter

Semrock Part Number: FF421/491/567/659/776-Di01-25x36

IDEX Part Number: FL-410491  /

Semrock
Multi-edge dichroics have two or more transitions from high reflectance to high transmission. Semrock offers a wide range of dichroic beamsplitters that exhibit steep edges with very high reflection and transmission bands. This standard epi-fluorescence dichroic beamsplitter is optimized only for broadband light sources such as LEDs or similar light engines.

BrightLine dichroic beamsplitter for DAPI/FITC/TRITC/Cy5/Cy7 imaging channels optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths.
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  • 867.83 USD Each
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Optical Specifications Value
Transmission Band 1 Tavg > 90% 426 – 456 nm, Tavg > 93% 426 – 456 nm (AOI = 45° ± 0°)
Transmission Band 2 Tavg > 90% 498 - 524 nm, Tavg > 93% 498 - 524 nm (AOI = 45° ± 0°)
Transmission Band 3 Tavg > 90% 574 – 611 nm, Tavg > 93% 574 – 611 nm (AOI = 45° ± 0°)
Transmission Band 4 Tavg > 90% 667 – 701 nm, Tavg > 93% 667 – 701 nm (AOI = 45° ± 0°)
Transmission Band 5 Tavg > 90% 784 – 850 nm, Tavg > 93% 784 – 850 nm (AOI = 45° ± 0°)
Reflection Band 1 Ravg > 95% 369 – 413 nm
Reflection Band 2 Ravg > 95% 471 – 483 nm
Reflection Band 3 Ravg > 95% 541 - 557 nm
Reflection Band 4 Ravg > 95% 630 – 647 nm
Reflection Band 5 Ravg > 95% 725 – 757 nm
Edge Wavelength 1 421 nm
Edge Wavelength 2 491 nm
Edge Wavelength 3 567 nm
Edge Wavelength 4 659 nm
Edge Wavelength 5 776 nm
General Filter Specifications Value
Angle of Incidence 45 ± 1.5 degrees
Cone Half-angle 2 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Flatness / RWE Classification   Standard Epi-fluorescence
Reflected Wavefront Error >> 6λ P-V RWE @ 632.8 nm
Steepness Standard
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Filter Thickness (unmounted) 1.05 mm
Filter Thickness Tolerance (unmounted) ± 0.05 mm
Orientation Reflective surface marked with part number - Orient in direction of incoming light

FF01-391/477/549/639/741-25

FL-410455 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 369 – 413 nm, Tavg > 90% 471 – 483 nm, Tavg > 90% 541 – 557 nm, Tavg > 90% 630 – 647 nm & Tavg > 90% 725 – 757 nm
  • Center Wavelengths of 391nm, 477 nm, 549 nm, 639 nm & 741 nm and GMBWs of 44 nm, 12 nm, 16 nm, 17 nm & 32 nm
  • Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths

FF01-441/511/593/684/817-25

FL-410484 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 426 – 456 nm, Tavg > 90% 498 – 524 nm, Tavg > 90% 574 – 611 nm, Tavg > 90% 667 – 701 nm & Tavg > 90% 784 – 850 nm
  • Center Wavelengths of 441nm, 511 nm, 592.5 nm, 684 nm & 817 nm and GMBWs of 30 nm, 26 nm, 37 nm, 34 nm & 66 nm
  • Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths

1 of 1

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Specifications

FF01-391/477/549/639/741-25

FL-410455 / 0

Out of Stock Backordered In Stock
  • Tavg > 85% 369 – 413 nm, Tavg > 90% 471 – 483 nm, Tavg > 90% 541 – 557 nm, Tavg > 90% 630 – 647 nm & Tavg > 90% 725 – 757 nm
  • Center Wavelengths of 391nm, 477 nm, 549 nm, 639 nm & 741 nm and GMBWs of 44 nm, 12 nm, 16 nm, 17 nm & 32 nm
  • Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths

FF01-441/511/593/684/817-25

FL-410484 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 426 – 456 nm, Tavg > 90% 498 – 524 nm, Tavg > 90% 574 – 611 nm, Tavg > 90% 667 – 701 nm & Tavg > 90% 784 – 850 nm
  • Center Wavelengths of 441nm, 511 nm, 592.5 nm, 684 nm & 817 nm and GMBWs of 30 nm, 26 nm, 37 nm, 34 nm & 66 nm
  • Optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm, 635 nm & 750 nm wavelengths

1 of 1

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.