441/511/717 nm BrightLine® triple-band bandpass filter

Semrock Part Number: FF01-441/511/717-25

IDEX Part Number: FL-412322  /

Semrock
Individual multiband fluorescence bandpass filters that utilize Semrock's patented single-substrate construction. These filters have extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands. All thin-film, hard-coated construction for unsurpassed performance and reliability.

BrightLine emitter for DAPI/GFP/mCherry imaging channels optimized for Lumencor CELESTA light engine® utilizing 408 nm, 473 nm, 545 nm wavelengths.
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Optical Specifications Value
Transmission Band 1 Tavg > 90% 426 – 456 nm
Transmission Band 2 Tavg > 90% 498 – 524 nm
Transmission Band 3 Tavg > 90% 584 – 850 nm
Blocking Band 1   ODavg > 4 200 – 275 nm (Design specification - measurements are noise-floor limited).
Blocking Band 2 ODavg > 4 275 – 369 nm
Blocking Band 3   ODavg > 8 369 – 413 nm (Design specification - measurements are noise-floor limited).
Blocking Band 4 OD > 3.5 420 nm
Blocking Band 5 OD > 3.5 463 nm
Blocking Band 6   ODavg > 8 471 – 483 nm (Design specification - measurements are noise-floor limited)
Blocking Band 7 OD > 3.5 491 nm
Blocking Band 8 OD > 3.5 532 nm
Blocking Band 10 OD > 3.5 572 nm
Blocking Band 9   ODavg > 8 541 – 560 nm (Design specification - measurements are noise-floor limited)
Center Wavelength 1 441 nm
Center Wavelength 2 511 nm
Center Wavelength 3 717 nm
Guaranteed Minimum Bandwidth 1 30 nm
Guaranteed Minimum Bandwidth 2 26 nm
Guaranteed Minimum Bandwidth 3 266 nm
FWHM Bandwidth 1 (nominal) 35 nm
FWHM Bandwidth 2 (nominal) 30 nm
FWHM Bandwidth 3 (nominal) 300 nm
General Filter Specifications Value
Angle of Incidence 0 ± 5 degrees
Cone Half-angle 7 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications   Value
Transverse Dimensions (Diameter) 25 mm
Transverse Tolerance (mounted) + 0.0 / – 0.1 mm
Filter Thickness (Mounted) 3.5 mm
Filter Thickness Tolerance (Mounted) ± 0.1 mm
Clear Aperture ≥ 22 mm
Scratch-Dig 60-40
Substrate Thickness (unmounted) 2.0 mm
Substrate Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Arrow on ring indicates preferred direction of propagation of light

FF421/491/572-Di01-25x36

FL-412324 / 0

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  • Dichroic Beamsplitter with edges at 421 nm, 491 nm & 572 nm
  • Ravg > 95% 350 – 413 nm, Ravg > 95% 471 – 483 nm & Ravg > 95% 541 – 560 nm
  • Tavg > 93% 426 – 456 nm, Tavg > 93% 498 – 524 nm & Tavg > 93% 584 – 850 nm

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TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical DensityTables to describe the relationship between OD and transmission with tips for quickly calculating OD
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical DensityTables to describe the relationship between OD and transmission with tips for quickly calculating OD
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets