400/16 nm Nanopede single-band bandpass filter

Semrock Part Number: FBP01-400/16-12.5

IDEX Part Number: FL-431025  /

Semrock

Individual fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. Pair this filter with other Nanopede filters to step through the spectrum in 20 nm Full-Width, Half Max (FWHM) steps. This value approach to filter design provides essential filter performance for detector-based applications.

This filter family is designed for scattered light and point detection applications only, for imaging applications, consider our BrightLine filters.
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Optical Specifications Value
Transmission Band 1 Tavg > 93% 392 – 408 nm
Blocking Band 1 ODavg > 5 330 – 386 nm
Blocking Band 2 OD > 5 445 nm
Blocking Band 3 OD > 5 488 nm
Blocking Band 4 OD > 5 532 nm
Blocking Band 5 OD > 5 633 nm
Blocking Band 6 ODavg > 5 415 – 900 nm
Center Wavelength 1 400 nm
Guaranteed Minimum Bandwidth 1 16 nm
FWHM Bandwidth 1 (nominal) 20 nm
General Filter Specifications Value
Angle of Incidence 0 ± 5 degrees
Cone Half-angle 7 degrees
Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications   Value
Transverse Dimensions (diameter) 12.5 mm
Transverse Tolerance + 0.0 / - 0.1 mm
Filter Thickness (mounted) 3.5 mm
Filter Thickness Tolerance (mounted) ± 0.1 mm
Clear Aperture ≥ 10 mm
Scratch-Dig 80-50
Filter Thickness (unmounted) 2.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Arrow on ring indicates preferred direction of propagation of light.
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Question 1: What is the FBP01-400/16-12.5 filter used for?

Answer: The FBP01-400/16-12.5 is a single-band optical bandpass filter that transmits light centered around 400 nm while blocking unwanted wavelengths. It is commonly used in fluorescence instruments, spectral flow cytometry, point-detection systems, and optical prototyping applications..

Question 2: What does "400/16" mean?

Answer: The designation "400/16" indicates- Center wavelength: 400 nm and Guaranteed maximum bandwidth (GMBW): 16 nm. The filter belongs to the Nanopede™ family, which is designed around 20 nm FWHM spectral spacing across the spectrum.:

Question 3: Is this filter suitable for fluorescence applications?

Answer: The filter family was specifically developed for fluorescence point-detection based systems that require spectral discrimination. This filter family is not suitable for imaging applications.

Question 4: What makes Nanopede filters unique?

Answer: Nanopede filters are designed to provide:

  • Cost-effective spectral coverage
  • Consistent 20 nm spacing across the spectrum
  • High transmission
  • Compact packaging

This allows engineers to rapidly build and evaluate systems without requiring premium filter sets for every channel.

Question 5: Can I combine this filter with other Nanopede filters?

Answer: Yes. Nanopede filters are intended to be used as a family. Multiple filters can be combined to step across the spectrum in adjacent wavelength bands, helping optimize spectral point-based detection systems.

Specifications

TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
LinkDescription
Request a DrawingLooking for an optical filter drawing file for this product? Request one now!
Request Custom Optical FiltersContact us today to start a conversation about custom optical filters.
Access Optical Filter WarrantyFind our warranty in our Terms & Conditions of Sale.
Semrock Optical Filters RoHS CertificateRoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances.
Semrock Optical Filters REACH CertificateREACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry.
Download Optical Filters 101 GuideFind an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now.
Request Clean Filter Checklist PosterLooking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster.

Question 1: What is the FBP01-400/16-12.5 filter used for?

Answer: The FBP01-400/16-12.5 is a single-band optical bandpass filter that transmits light centered around 400 nm while blocking unwanted wavelengths. It is commonly used in fluorescence instruments, spectral flow cytometry, point-detection systems, and optical prototyping applications..

Question 2: What does "400/16" mean?

Answer: The designation "400/16" indicates- Center wavelength: 400 nm and Guaranteed maximum bandwidth (GMBW): 16 nm. The filter belongs to the Nanopede™ family, which is designed around 20 nm FWHM spectral spacing across the spectrum.:

Question 3: Is this filter suitable for fluorescence applications?

Answer: The filter family was specifically developed for fluorescence point-detection based systems that require spectral discrimination. This filter family is not suitable for imaging applications.

Question 4: What makes Nanopede filters unique?

Answer: Nanopede filters are designed to provide:

  • Cost-effective spectral coverage
  • Consistent 20 nm spacing across the spectrum
  • High transmission
  • Compact packaging

This allows engineers to rapidly build and evaluate systems without requiring premium filter sets for every channel.

Question 5: Can I combine this filter with other Nanopede filters?

Answer: Yes. Nanopede filters are intended to be used as a family. Multiple filters can be combined to step across the spectrum in adjacent wavelength bands, helping optimize spectral point-based detection systems.