The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers for advanced microscopy. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.
Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.
1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.
1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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660.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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LL01-785-12.5
FL-007933 / 0
- Laser Wavelength = 785 nm
- Tabs > 90% 785 nm
- FWHM of 3.0 nm (typical); 5.5 nm (maximum)
BLP01-785R-25
FL-008534 / 0
- Filter with 50% edge at 805 nm
- 309 cm-1 transition
- Tavg > 93% from 812.9 – 1200 nm
NF03-785E-25
FL-009358 / 0
- Laser Wavelength = 785 nm
- Typical Notch Bandwidth = 39 nm
- ODabs > 6 785 nm
LPD02-785RU-25
FL-008049 / 0
- Laser Wavelength = 785 nm
- 126 cm-1 transition
- Tavg > 93% 795.2 – 1213.8 nm
- Flatness / RWE: Laser
LD01-785/10-12.5
FL-007686 / 0
- Laser Wavelength = 785 nm
- Tabs > 90% 780 – 790 nm
- Center Wavelength of 785 nm and GMBW of 10 nm
NFD01-785-25x36
FL-009360 / 0
- Laser Wavelength = 785 nm
- R > 98% 785 nm
- Tavg > 90% 350 – 749 nm & 841 – 1600 nm
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Flatness of Dichroic Beamsplitters | Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity |
| Laser Damage Threshold | Provides information for calculating laser damage threshold for pulsed and continuous wave lasers |
| Optical Filters for Laser-based Fluorescence Microscopes | Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
Journal Article
| Topic | Description |
| Bringing Super-resolution to Fluorescence Microscopy | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
| Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
| Perfecting TIRF Optics | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
White Paper
| Topic | Description |
| Optical Filters for Laser-based Fluorescence Microscopes | Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems. |
| Flatness of Dichroic Beamsplitters Affects Focus and Image Quality | Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy. |
| Super-resolution Microscopy | Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
Application
| Topic | Description |
| TIRF (Total Internal Reflection Fluorescence) | An overview of TIRF microscopy, including solutions from Semrock and additional learning resources. |
| Laser Scanning/Spinning Disk Confocal Microscopy | An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources. |
| Laser-based Instrumentation | An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources. |