405/488/561/635 nm lasers BrightLine® quad-edge super-resolution / TIRF dichroic beamsplitter

Semrock Part Number: Di03-R405/488/561/635-t3-25x36

IDEX Part Number: FL-007018  /

Semrock

 The perfect beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally, they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE

λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE

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Optical Specifications Value
Transmission Band 1 Tavg > 93% 426 – 462 nm
Transmission Band 2 Tavg > 93% 502.5 – 544.5 nm
Transmission Band 3 Tavg > 93% 582 – 617.5 nm
Transmission Band 4 Tavg > 93% 663 – 1200 nm
Reflection Band 1 Rabs > 94% 370 – 410 nm
Reflection Band 1 (p-pol) Rabs > 90% 370 – 410 nm
Reflection Band 1 (s-pol) Rabs > 98% 370 – 410 nm
Reflection Band 2 Rabs > 94% 473 – 491 nm
Reflection Band 2 (p-pol) Rabs > 90% 473 – 491 nm
Reflection Band 2 (s-pol) Rabs > 98% 473 – 491 nm
Reflection Band 3 Rabs > 94% 559 – 568.2 nm
Reflection Band 3 (p-pol) Rabs > 90% 559 – 568.2 nm
Reflection Band 3 (s-pol) Rabs > 98% 559 – 568.2 nm
Reflection Band 4 Rabs > 94% 632.8 – 647.1 nm
Reflection Band 4 (p-pol) Rabs > 90% 632.8 – 647.1 nm
Reflection Band 4 (s-pol) Rabs > 98% 632.8 – 647.1 nm
Reflection Band 5 Ravg > 90% 350 – 370 nm
Edge Wavelength 1 418.5 nm
Edge Wavelength 2 498.3 nm
Edge Wavelength 3 576.5 nm
Edge Wavelength 4 658.1 nm
Laser Wavelengths 1 375 +/- 3 nm, 405 +/- 5 nm
Laser Wavelengths 2 473 +2/-0 nm, 488 +3/-2 nm
Laser Wavelength 3 559 +5/-0 nm, 561.4 nm, 568.2 nm
Laser Wavelengths 4 632.8 nm, 635 +7/-0 nm, 647.1 nm
General Filter Specifications Value
Angle of Incidence 45 degrees with a shift of 0.35%/degree (40 – 50 degrees)
Cone Half-angle 0.5 degrees
Optical Damage Rating 1 J/cm² @ 532 nm (10 ns pulse width)
Reflected Wavefront Error < 0.2λ P-V RWE @ 632.8 nm
Steepness Steep
Flatness / RWE Classification   Super-resolution / TIRF
Physical Filter Specifications   Value
Transverse Dimensions (L x W) 25.2 mm x 35.6 mm
Transverse Tolerance ± 0.1 mm
Clear Aperture ≥ 80% (elliptical)
Scratch-Dig 60-40
Substrate Type Fused Silica
Filter Thickness (unmounted) 3.0 mm
Filter Thickness Tolerance (unmounted) ± 0.1 mm
Orientation Reflective surface marked with laser dot - Orient in direction of incoming light

Specifications