The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers for advanced microscopy. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.
Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.
1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.
1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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725.73 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Flatness of Dichroic Beamsplitters | Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity |
| Laser Damage Threshold | Provides information for calculating laser damage threshold for pulsed and continuous wave lasers |
| Multiband Filter Set Terminology | Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets |
| Optical Filters for Laser-based Fluorescence Microscopes | Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
Journal Article
| Topic | Description |
| Bringing Super-resolution to Fluorescence Microscopy | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
| Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
| Perfecting TIRF Optics | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
White Paper
| Topic | Description |
| Optical Filters for Laser-based Fluorescence Microscopes | Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems. |
| Flatness of Dichroic Beamsplitters Affects Focus and Image Quality | Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy. |
| Super-resolution Microscopy | Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
Application
| Topic | Description |
| TIRF (Total Internal Reflection Fluorescence) | An overview of TIRF microscopy, including solutions from Semrock and additional learning resources. |
| Laser Scanning/Spinning Disk Confocal Microscopy | An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources. |
| Laser-based Instrumentation | An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 1 products from all pages.
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Flatness of Dichroic Beamsplitters | Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity |
| Laser Damage Threshold | Provides information for calculating laser damage threshold for pulsed and continuous wave lasers |
| Multiband Filter Set Terminology | Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets |
| Optical Filters for Laser-based Fluorescence Microscopes | Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
Journal Article
| Topic | Description |
| Bringing Super-resolution to Fluorescence Microscopy | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
| Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
| Perfecting TIRF Optics | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
White Paper
| Topic | Description |
| Optical Filters for Laser-based Fluorescence Microscopes | Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems. |
| Flatness of Dichroic Beamsplitters Affects Focus and Image Quality | Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy. |
| Super-resolution Microscopy | Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
Application
| Topic | Description |
| TIRF (Total Internal Reflection Fluorescence) | An overview of TIRF microscopy, including solutions from Semrock and additional learning resources. |
| Laser Scanning/Spinning Disk Confocal Microscopy | An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources. |
| Laser-based Instrumentation | An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Access Optical Filter Warranty | Find our warranty in our Terms & Conditions of Sale. |
| Semrock Optical Filters RoHS Certificate | RoHS, stands for Restriction of Hazardous Substances. The current RoHS Directive currently restricts the use of ten substances. |
| Semrock Optical Filters REACH Certificate | REACH is a regulation of the European Union, adopted to improve the protection of human health and the environment from the risks that can be posed by chemicals, while enhancing the competitiveness of the EU chemicals industry. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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