The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including newer all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the laser wavelengths, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light. This laser dichroic beamsplitter is optimized for reflecting laser beams up to 2.5 mm in diameter while minimizing RWE.
Interested in an improved Flatness / RWE version? Consider Semrock's Super-resolution / TIRF dichroics:
1λ P-V RWE on 1mm Di03-R594-t1 , optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3mm Di03-R594-t3, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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505.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
BLP01-594R-25
FL-008542 / 0
- Filter with 50% edge at 607 nm
- 411 cm-1 transition
- Tavg > 93% from 610.9 – 900 nm
NF03-594E-25
FL-009340 / 0
- Laser Wavelength = 594.1 nm
- Typical Notch Bandwidth = 21.8 nm
- ODabs > 6 594.1 nm
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Flatness of Dichroic Beamsplitters | Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity |
| Laser Damage Threshold | Provides information for calculating laser damage threshold for pulsed and continuous wave lasers |
| Optical Filters for Laser-based Fluorescence Microscopes | Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
Journal Article
| Topic | Description |
| Bringing Super-resolution to Fluorescence Microscopy | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
| Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
| Perfecting TIRF Optics | Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection |
White Paper
| Topic | Description |
| Optical Filters for Laser-based Fluorescence Microscopes | Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems. |
| Flatness of Dichroic Beamsplitters Affects Focus and Image Quality | Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy. |
| Super-resolution Microscopy | Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope |
Application
| Topic | Description |
| TIRF (Total Internal Reflection Fluorescence) | An overview of TIRF microscopy, including solutions from Semrock and additional learning resources. |
| Laser Scanning/Spinning Disk Confocal Microscopy | An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources. |
| Laser-based Instrumentation | An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources. |
total selected
Clear selection
Select all 4 products from all pages.
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
BLP01-594R-25
FL-008542 / 0
- Filter with 50% edge at 607 nm
- 411 cm-1 transition
- Tavg > 93% from 610.9 – 900 nm
NF03-594E-25
FL-009340 / 0
- Laser Wavelength = 594.1 nm
- Typical Notch Bandwidth = 21.8 nm
- ODabs > 6 594.1 nm