This unique "flamethrower" filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For most applications that require minimized photobleaching, we recommend our DAPI-11LP-A set. In all cases our filters are uniquely hard-coated, for "no burn-out" performance where it is most needed.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,485.96 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-377/50-25
FL-004661 / 0
- Tavg > 85% 352 – 402 nm
- Center Wavelength of 377 nm and GMBW of 50 nm
- FWHM of 53.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
FF02-409/LP-25
FL-009097 / 0
- Filter with 50% edge at 415 nm
- Tavg > 93% 417 – 1100 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
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| 4-methylumbelliferone | 357 | 441 |
|
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| 7-amino-4-methylcoumarin (AMC) | 346 | 442 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Alexa Fluor® 350 | 343 | 441 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| Calcofluor White | 351 | 444 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| ChlorophyllA | 480 | 650 |
|
|
| DAPI | 359 | 461 |
|
|
| Dapoxyl | 373 | 579 |
|
|
| DEAC | 432 | 472 |
|
|
| DiFMU-pH 9.0 | 358 | 450 |
|
|
| DY-415 | 418 | 470 |
|
|
| DyLight 350 | 351 | 434 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| Fluoro-Gold™ | 365 | 418 |
|
|
| FluoSpheres Blue fluorescent microspheres | 356 | 414 |
|
|
| Fura Red™ (high pH) | 572 | 657 |
|
|
| Fura-2 (calcium bound) | 336 | 505 |
|
|
| Fura-2 (calcium free) | 366 | 515 |
|
|
| GFPuv | 395 | 509 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| Hoechst 33258 | 352 | 455 |
|
|
| Hoechst 33342 | 352 | 455 |
|
|
| Hoechst 34580 | 392 | 440 |
|
|
| Hypericin | 600 | 603 |
|
|
| indo-1 | 346 | 479 |
|
|
| LIVE-DEAD Fixable Aqua Dead Cell Stain | 367 | 526 |
|
|
| LIVE-DEAD Fixable Blue Dead Cell Stain | 343 | 441 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| LysoTracker Blue | 373 | 422 |
|
|
| Marina Blue | 363 | 461 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| Pacific Orange | 400 | 551 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| Quinine sulfate | 349 | 461 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| Sirius | 355 | 424 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SYPRO Ruby protein gel stain | 469 | 631 |
|
|
| TagBFP | 402 | 457 |
|
|
| ThiolTracker Violet+GSH | 404 | 526 |
|
|
| Vybrant DyeCycle Violet | 369 | 437 |
|
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-377/50-25
FL-004661 / 0
- Tavg > 85% 352 – 402 nm
- Center Wavelength of 377 nm and GMBW of 50 nm
- FWHM of 53.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
FF02-409/LP-25
FL-009097 / 0
- Filter with 50% edge at 415 nm
- Tavg > 93% 417 – 1100 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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FITC-Di01-Clin-25x36
FL-007186