This unique "flamethrower" filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For most applications that require minimized photobleaching, we recommend our DAPI-11LP-A set. In all cases our filters are uniquely hard-coated, for "no burn-out" performance where it is most needed.
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||21.8 mm||5.0 mm|
|Emitter (Diameter)||23.3 mm||5.0 mm|
|Dichroic (L × W)||22.0 mm × 29.0 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|