BrightLine^® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-5060C-OFX

IDEX Part Number: S-000181 /

Filter Role	Filter Size	Maximum Thickness
Exciter (Diameter)	32.0 mm	5.0 mm
Emitter (Diameter)	32.0 mm	3.5 mm
Dichroic (L × W)	32.0 mm × 44.0 mm (Corners must me cut for OFX cube)	1.5 mm

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-amino-4-methylcoumarin (AMC)	346	442
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor^® 350	343	441
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DEAC	432	472
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 350	351	434
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
FluoSpheres Blue fluorescent microspheres	356	414
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
Hoechst 33342	352	455
Hoechst 34580	392	440
indo-1	346	479
LIVE-DEAD Fixable Blue Dead Cell Stain	343	441
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
Quinine sulfate	349	461
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Filter Role	Filter Size	Maximum Thickness
Exciter (Diameter)	32.0 mm	5.0 mm
Emitter (Diameter)	32.0 mm	3.5 mm
Dichroic (L × W)	32.0 mm × 44.0 mm (Corners must me cut for OFX cube)	1.5 mm

Standard Component Sizes