Brightest DAPI set on the market. This unique "flamethrower" filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times this set exhibits up to 5 times more brightness but has lower contrast and more photobleaching than the set.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,464.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-377/50-25
FL-004661 / 0
- Tavg > 85% 352 – 402 nm
- Center Wavelength of 377 nm and GMBW of 50 nm
- FWHM of 53.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-amino-4-methylcoumarin (AMC) | 346 | 442 |
|
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Alexa Fluor® 350 | 343 | 441 |
|
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| Alexa Fluor® 405 | 401 | 422 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| ATTO 390 | 392 | 477 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BFP (EBFP) | 380 | 440 |
|
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| Calcofluor White | 351 | 444 |
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| Cascade Blue™ | 401 | 419 |
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| CellTrace calcein violet | 400 | 452 |
|
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| DAPI | 359 | 461 |
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| DEAC | 432 | 472 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
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| DY-415 | 418 | 470 |
|
|
| DyLight 350 | 351 | 434 |
|
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| DyLight 405 | 398 | 420 |
|
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| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| FluoSpheres Blue fluorescent microspheres | 356 | 414 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| Hoechst 33258 | 352 | 455 |
|
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| Hoechst 33342 | 352 | 455 |
|
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| Hoechst 34580 | 392 | 440 |
|
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| indo-1 | 346 | 479 |
|
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| LIVE-DEAD Fixable Blue Dead Cell Stain | 343 | 441 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
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| LysoSensor Blue | 374 | 424 |
|
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| LysoTracker Blue | 373 | 422 |
|
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| Marina Blue | 363 | 461 |
|
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| mBBr+GSH | 394 | 490 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| Quinine sulfate | 349 | 461 |
|
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| sgBFP™ | 387 | 451 |
|
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| Sirius | 355 | 424 |
|
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| SpectrumBlue | 405 | 449 |
|
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| TagBFP | 402 | 457 |
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| Vybrant DyeCycle Violet | 369 | 437 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-447/60-25
FL-004651 / 0
- Tavg > 93% 417 – 477 nm
- Center Wavelength of 447 nm and GMBW of 60 nm
- FWHM of 65.3 nm
FF01-377/50-25
FL-004661 / 0
- Tavg > 85% 352 – 402 nm
- Center Wavelength of 377 nm and GMBW of 50 nm
- FWHM of 53.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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