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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-5060C-NQF

IDEX Part Number: S-000176  /

Semrock

Brightest DAPI set on the market. This unique "flamethrower" filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times this set exhibits up to 5 times more brightness but has lower contrast and more photobleaching than the set.

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  • Price Not Available.
  • 1,464.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-amino-4-methylcoumarin (AMC) 346 442
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BFP (EBFP) 380 440
Calcofluor White 351 444
Cascade Blue™ 401 419
CellTrace calcein violet 400 452
DAPI 359 461
DEAC 432 472
DiFMU-pH 9.0 358 450
DY-415 418 470
DyLight 350 351 434
DyLight 405 398 420
ER-Tracker™ Blue-White DPX 372 557
FluoSpheres Blue fluorescent microspheres 356 414
HiLyte Fluor™ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
indo-1 346 479
LIVE-DEAD Fixable Blue Dead Cell Stain 343 441
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
Quinine sulfate 349 461
sgBFP™ 387 451
Sirius 355 424
SpectrumBlue 405 449
TagBFP 402 457
Vybrant DyeCycle Violet 369 437

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)

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