This filter set is optimized for minimized photobleaching due to the carefully optimized narrow, red-shifted exciter filter. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources. For specialized applications where brightness trumps contrast, use our DAPI-50LP-A set, which has the highest brightness on the market.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,573.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
FF02-409/LP-25
FL-009097 / 0
- Filter with 50% edge at 415 nm
- Tavg > 93% 417 – 1100 nm
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
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| 4-methylumbelliferone | 357 | 441 |
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| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
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| Alexa Fluor® 350 | 343 | 441 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
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| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| ATTO 390 | 392 | 477 |
|
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| BD Horizon V450 | 406 | 449 |
|
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| BD Horizon V500 | 410 | 500 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| Calcofluor White | 351 | 444 |
|
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| Cascade Blue™ | 401 | 419 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| ChlorophyllA | 480 | 650 |
|
|
| DAPI | 359 | 461 |
|
|
| Dapoxyl | 373 | 579 |
|
|
| DEAC | 432 | 472 |
|
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| DiFMU-pH 9.0 | 358 | 450 |
|
|
| DY-415 | 418 | 470 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| ECFP | 435 | 475 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| Fura Red™ (high pH) | 572 | 657 |
|
|
| Fura-2 (calcium free) | 366 | 515 |
|
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| GFPuv | 395 | 509 |
|
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| HiLyte Fluor™ 405 | 403 | 428 |
|
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| Hoechst 33258 | 352 | 455 |
|
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| Hoechst 33342 | 352 | 455 |
|
|
| Hoechst 34580 | 392 | 440 |
|
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| LIVE-DEAD Fixable Aqua Dead Cell Stain | 367 | 526 |
|
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| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Blue | 374 | 424 |
|
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| LysoTracker Blue | 373 | 422 |
|
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| Marina Blue | 363 | 461 |
|
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| mBBr+GSH | 394 | 490 |
|
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| Pacific Blue™ | 404 | 455 |
|
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| Pacific Orange | 400 | 551 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| sgBFP™ | 387 | 451 |
|
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| Sirius | 355 | 424 |
|
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| SpectrumBlue | 405 | 449 |
|
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| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| TagBFP | 402 | 457 |
|
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| ThiolTracker Violet+GSH | 404 | 526 |
|
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| Vybrant DyeCycle Violet | 369 | 437 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-387/11-25
FL-004663 / 0
- Tavg > 90% 382 – 393 nm
- Center Wavelength of 387.5 nm and GMBW of 11 nm
- FWHM of 14.8 nm
FF409-Di03-25x36
FL-007109 / 0
- Dichroic Beamsplitter with edge at 409 nm
- Ravg > 98% 327 – 404 nm
- Tavg > 93% 415 - 950 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
FF02-409/LP-25
FL-009097 / 0
- Filter with 50% edge at 415 nm
- Tavg > 93% 417 – 1100 nm
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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