BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

Semrock Part Number: DAPI-11LP-A-NQF

IDEX Part Number: S-001407  /

Semrock

This filter set is optimized for minimized photobleaching due to the carefully optimized narrow, red-shifted exciter filter. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources. For specialized applications where brightness trumps contrast, use our DAPI-50LP-A set, which has the highest brightness on the market.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,464.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FF02-409/LP-25

FL-009097 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 415 nm
  • Tavg > 93% 417 – 1100 nm

1 of 1

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
AMCA (Aminomethylcoumarin) 350 448
ATTO 390 392 477
BD Horizon V450 406 449
BD Horizon V500 410 500
BFP (EBFP) 380 440
Calcofluor White 351 444
Cascade Blue™ 401 419
Cascade Yellow™ 399 549
CellTrace calcein violet 400 452
CellTracker Violet BMQC+GSH 406 526
ChlorophyllA 480 650
DAPI 359 461
Dapoxyl 373 579
DEAC 432 472
DiFMU-pH 9.0 358 450
DY-415 418 470
DyLight 405 398 420
ECFP 435 475
ecliptic pHluorin pH5.5 473 507
ER-Tracker™ Blue-White DPX 372 557
Fura Red™ (high pH) 572 657
Fura-2 (calcium free) 366 515
GFPuv 395 509
HiLyte Fluor™ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Aqua Dead Cell Stain 367 526
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
Lucifer yellow 428 544
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
Pacific Orange 400 551
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
sgBFP™ 387 451
Sirius 355 424
SpectrumBlue 405 449
SYPRO Ruby protein gel stain 469 631
TagBFP 402 457
ThiolTracker Violet+GSH 404 526
Vybrant DyeCycle Violet 369 437

Related Products

FF01-387/11-25

FL-004663 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 381.5 – 392.5 nm
  • Center Wavelength of 387 nm and GMBW of 11 nm
  • FWHM of 14.8 nm

FF409-Di03-25x36

FL-007109 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 409 nm
  • Ravg > 98% 327 – 404 nm
  • Tavg > 93% 415 - 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

Custom sizing delivery confirmed ARO (sizing fee applies).

FF02-409/LP-25

FL-009097 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 415 nm
  • Tavg > 93% 417 – 1100 nm

1 of 1

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm

1.1 mm
(Can accept a 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.