Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.
This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.5 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?|